Methods for high-throughput glycoproteomic analysis - HTPGlycomet

The main aim of the HTP-GlycoMet proposal is to develop technologies, which will enable high-throughput analysis of glycosylation of individual proteins from body fluids and cell membranes and apply them to understand some key processes in immunity and infections. Glycan analysis is extremely demanding from both the technological and the conceptual aspect and (beside one study performed by partners in this proposal) large-scale studies of glycosylation of individual proteins have not been attempted previously. However, by successfully combining complementary expertise in the (i) production of specialised monolithic chromatographic tools for high-throughput fractionation of complex biological fluids, (ii) purification of proteins from body fluids and membrane proteins, (iii) high-throughput glycomic analysis by use of chromatography and multiplexed capillary gel electrophoresis and (iv) expertise in the field of viral immunology the consortium aims to achieve significant progress in this direction. All partners in this consortium (coming from the University of Rijeka, Faculty of Medicine of the University of Rijeka, Croatia, Genos Zagreb, Croatia, Bia Separations Ajdovščina, Slovenia, Max Planck Society, Magdeburg, Germany) are recognized leaders who have already made significant progress beyond the state of the art in their respective fields. Through the HTP-GlycoMet project they will organise secondments to capitalize on synergistic effects of this interdisciplinary and transnational collaboration. The recruited researcher will work on establishment of new protein expression systems, in order to support protein purification, protein characterization and quality control.