Thomas Klarić, PhD - Researcher

Education

2006-2012 Ph.D. University of Adelaide Ph.D. conferred July 31st 2012
2005 Honours University of Adelaide First Class Honours
2002-2004 Undergraduate University of Adelaide Bachelor of Biotechnology, GPA = 5.4

Research experience

2012-2014 Post-doctoral researcher, Koblar laboratory
Stroke Research Programme, University of Adelaide
2006-2012 Ph.D. under the supervision of Prof. Simon Koblar
Project entitled: “Expression and function of Npas4 during early development”
School of Molecular and Biomedical Science, University of Adelaide
2005 Honours project under the supervision of Prof. Simon Koblar
Project entitled: “The role of Nxf in neurogenesis”
School of Molecular and Biomedical Science, University of Adelaide

Laboratory skills

Molecular biology: RT-PCR, qRT-PCR, immunoblotting, Southern blotting, in situ hybridisation, cloning, immunohistochemistry, flow cytometry
Tissue culture: Mammalian cell lines and primary cultures (adult neural stem cells; both adherent cultures and neurospheres), transient transfection and stable transduction using lentiviral delivery
Animal work: Mouse husbandry, genotyping, behaviour testing, embryo dissections, mouse photochemical stroke model
Microscopy/Imaging: Fluorescence microscopy
Photonics: Implantation of fibre optic sensors into the rodent brain

Teaching experience

2006-2014 Assisted in supervision of Honours and Ph.D. students, including demonstration of laboratory techniques and assistance with experimental design

Publications

“A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells” Stem Cell Research and Therapy, 2014
Klarić T, Thomas P, Dottori M, Leong W, Koblar S, Lewis M.
“Upregulation of the neuronal Per-Arnt-Sim domain protein 4 (Npas4) in the rat corticolimbic system following focal cerebral ischemia” EJN, 2013
Leong W, Klarić T, Lin Y, Lewis M, Koblar S.

Grants
2013 Institute of Photonics and Advanced Sensing (IPAS) Extending Collaborations Pilot Project
Project Title: Real-time characterisation of photochemically-induced ischaemic damage in a mouse model of subcortical stroke using fibre-optic sensors.

Awards and scholarships

2013 Robinson Institute Early Career Researcher International Travel Grant ($1,800)
2012 ATA Scientific Travel Award ($400)
2008 Faculty of Health Sciences Postgraduate Travelling Fellowship ($1000)
2008 Centre for the Molecular Genetics of Development (CMGD) Travel Support ($1500)
2008 Network in Genes and Environment in Development (NGED) Conference Participation Award ($1000)
2008 Genetics Society of Australasia (GSA) Meeting Poster Prize - 3rd place ($50)
2008 Faculty of Health Sciences Research Secretariat Photo Competition - 2nd place ($50)
2007 School of Molecular and Biomedical Sciences Images Competition - Runner up ($50)
2007 Australian Stem Cell Centre (ASCC) Conference and Travel Grant ($650)
2006 University of Adelaide Faculty of Sciences Divisional Scholarship